Preparation of different types of media – PDA, SDA, RBA, YGC Agar
Isolation of fungus from different sources.
Microscopic – LPCB, General microscopic & Slide vculture techniques
Antifungal assay
Screening for extracellular enzyme production
Detection of Endophytic fungi
Maintenance of fungal cultures
Immunology
ODD Pattern and Titration – Double Immuno Diffusion Assay
SRID – Single Immuno Diffusion Assay
Rocket electrophoresis
Counter current Immuno Electrophoresis
Immuno Electrophoresis
Serum protein electrophoresis
Enzyme Technology
Screening of potent Enzyme producers – Plate assay
Formulation concepts for production media – Strain improvement
Optimization strategies for enzyme production
Methods for quantification of enzymes
Growth kinetics for enzyme producers
The concepts of Enzyme harvesting concepts
UNDERSTANDING MICROBIOLOGY FOR NON MICROBIOLOGISTS
Microorganism – types and diversity and nutritional requirements for growth
Case study exercise: diagnosis of food microbiological problems
Hygiene & environmental sampling in the factory
Food poisoning
Food spoiling
Growth inhibition & destruction of microbes of toxics
Factory affecting growth & survival of microorganisms
PCR & REAL TIME PCR TECHNIQUES AND ITS APPLICATIONS
Basic Microbial techniques and their storage – Culturing methods, Microbial isolation techniques, Media optimisation, Storing of Microorganisms
Basic Microbial and Molecular techniques – Microbial culture techniques, Growth curve analysis, Nucleic acid extraction, Electrophoresis separation, Polymerase chain reaction (PCR), Primer designing and standardisation
Microbial Gene Expression studies – Microbial nucleic acid extraction and Quantification, Primer designing and standardisation, Real Time PCR: Gene Expression studies, Result Interpretation
Human Gene Expression studies – Human nucleic acid extraction and Quantification, PCR, Primer designing and standardisation, Result interpretation
BACTERIAL CLONING TECHNIQUE
Media preparation and vector selection
Bacteria culture and Maintenance
Preparation of Competent cells
Restriction digestion
Ligation and Transformation
Small scale isolation of plasmid DNA
Large scale isolation of plasmid DNA
Restriction digestion
Ligation
Gel Electrophoresis
Elution of DNA from gel
Expression studies
PROTEOMICS
Culture maintenance
Extraction and estimation of protein
Purification of protein
SDS-PAGE
Staining
Western Blot
Western Blot visualization
CELL CULTURE - TECHNICAL TRAINING
Introduction of basic components of Cell culture and environment
Media preparation
Sterile technique and contamination control
Specific cell types and their requirement
Culture of cells in attachment and suspension and scaling up of cultures
Passaging of cells
Study of invitro cell growth behaviour
Determination of cell growth
Cell viability and cell proliferation assay techniques
Cryopreservation and retrieval of cells from frozen storage
FUNCTIONAL STUDIES RELATED TO CANCER
Soft agar assay
Clonogenic assay
Wound healing assay
Proliferation assay
Invasion/Migration assay
Angiogenesis
DNA DAMAGE AND REPAIR ASSAYS
DNA damage marker studies by western blot
DNA fragmentation
Comet assay
Pulse field gel electrophoresis
Halo assay
BIO-COMPUTING
Drug Designing
Protein Structure Prediction
Sequence analysis
Clinical SAS
Nucleotide Database Search
Nucleotide Sequence Retrieval
Sequence Alignment
Primer Designing
Phylogenetic Tree Construction
Protein Sequence Retrieval
RASMOL – Protein Structure Visualization tool
sbdbv – Swiss-Pdb Viewer
Motif scan and Identification
Hex – Docking
ExPASy
ISIS Draw – Structure Drawing
NCBI Literature Search
SPECIAL TECHNIQUES
Cell line revival, culture and storage methods
Analysis of gene expression across cell lines by semi-quantitative RT-PCR
Analysis of gene expression across cell lines by quantitative RT-PCR
Killing curve assay for eukaryotic selection markers
Transient gene silencing in cell line by liposome mediated SiRNA transfection
Analysis of the impact of gene expression in human cell line
Transient exogenous gene expression in human cell line
Engineering of human cell line to continuously express the gene on interest
Stable gene silencing by shRNA plasmid transfection/transduction
Analysis of protein expression in cell lines by Western blotting.
Gene over expression in human cells by reverse transfection.
Analysis of signalling pathway activity in human cell lines by dual luciferase reporter assay
Impact of modulation of gene expression on signalling pathway.
Analysis of anchorage independent growth property of human cells.
Determination of in-vitro toxicity of a compound on a call line.
Investigation of the epigenomic repression of a gene expression in cell line.
Analysis of cellular differentiation in human cell line.
Elucidation of the transcriptional regulation of gene promoters by reporter assay.
Determination of transcription factor-DNA interactions in human cells by Chromatin Immunoprecipitation (ChIP) assay.
Investigation of the epigenetic modification of a genomic region by bisulfite methylation assay.
Analysis of cellular apoptosis by flow cytometer/Western blot
Cell cycle phase analysis of human cell lines by flow cytometer.
Investigation of the cellular localization of the protein of interest by immunostaning.
PHYTOMEDICINE & ETHNOPHARMACOLOGY
Basics about herbal medicines and its significance in research
Preparing plant samples for extraction using different solvent systems
Overview of extraction of active components using different instrument s and techniques
Analysing metabolites using different chromatographic techniques .
Interpreting analytical data using different software solutions and plant metabolomics platforms
ADVANCED MOLECULAR MICROBIOLOGY
Good laboratory practices (GLP) in microbiology laboratories
Concept of basic calculation, solution preparation, media preparation.
Standard operating procedure (SOP) and application of instruments (Autoclave, pH meter, laminar air flow, colony counter, incubator and microscope).
Molecular Identification and characterization of microorganisms by PCR.
Sequence analysis by insilico tools
ADVANCED MOLECULAR BIOLOGY
Extraction, purification and quantification of DNA from Bacteria/Blood/Tissue
Designing and validation of primer – in silico analysis
PCR amplification and visualization of DNA fragments by Agarose gel electrophoresis
Extraction and Quantification of Proteins
Separation of proteins by SDS-PAGE
Visualization of proteins by CBB staining
ADVANCED BIOINFORMATICS AND CHEMINFORMATICS
Molecular Identification of Bacteria, Fungi, Actinomycetes and Algae, DNA isolation, PCR Amplification, Sequencing, Phylogeny tree and GenBank Submission with Results photos and Descriptions.
Biochemical and enzymatic characterization of Bacteria
Isolation, Characterization and molecular identification of probiotics from various sources.
Antibacterial activity (Five pathogens) in triplicate
Antifungal activity (One pathogens) intriplicate
Minimum inhibitory concentration
Minimum bactericidal activity
Antibiotic Susceptibility test (12 Antibiotics)
Enzymatic assay – Beta lactamase, Amylase, Lipase and Protease.
MOLECULAR MICROBIOLOGY TECHNIQUES
Molecular Identification of Bacteria, Fungi, Actinomycetes and Algae, DNA isolation, PCR Amplification, Sequencing, Phylogeny tree and GenBank Submission with Results photos and Descriptions.
Biochemical and enzymatic characterization of Bacteria
Isolation, Characterization and molecular identification of probiotics from various sources.
Antibacterial activity (Five pathogens) in triplicate
Antifungal activity (One pathogens) intriplicate
Minimum inhibitory concentration
Minimum bactericidal activity
Antibiotic Susceptibility test (12 Antibiotics)
Enzymatic assay – Beta lactamase, Amylase, Lipase and Protease.
